Review



dmao pi bacterial live  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Beyotime dmao pi bacterial live
    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. <t>(J)</t> <t>Live/dead</t> staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
    Dmao Pi Bacterial Live, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc13011198-324-5-10
    Average 99 stars, based on 226 article reviews
    dmao pi bacterial live - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds"

    Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.043

    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. (J) Live/dead staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
    Figure Legend Snippet: Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. (J) Live/dead staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

    Techniques Used: Biomarker Discovery, Staining, Activity Assay

    pH Self-Adaptive Antioxidant Capacity of HCOC (Stage II: anti-inflammation). Cu ion release behavior of (A) HC (1 mg/mL) and (B) HCOC (1 mg/mL) at different pH levels. (C) ABTS + and (D) H 2 O 2 scavenging activity at different pH of Cu 5.4 O, HC and HCOC. (E) O 2 ∙ - , (F)∙OH scavenging activity of Cu 5.4 O, HAs, HC, HCOC. (G) SOD-like, (H) CAT-like and (I) GPx-like activities of HCOC. (J) Fluorescence images showing intracellular ROS detection by DCFH-DA staining, live/dead staining images and (K) cell viability of L929 cells with different treatments (All groups received 500 μM H 2 O 2 and different HCOC concentrations (I: PBS; II: 0; III: 0.25; IV: 0.50; V: 1.0 mg/mL HCOC). (L) Quantitative analysis of the cells under different treatments. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001) (M) Schematic illustration of pH-responsive activity and ROS scavenging and alleviating cellular oxidative stress of HCOC.
    Figure Legend Snippet: pH Self-Adaptive Antioxidant Capacity of HCOC (Stage II: anti-inflammation). Cu ion release behavior of (A) HC (1 mg/mL) and (B) HCOC (1 mg/mL) at different pH levels. (C) ABTS + and (D) H 2 O 2 scavenging activity at different pH of Cu 5.4 O, HC and HCOC. (E) O 2 ∙ - , (F)∙OH scavenging activity of Cu 5.4 O, HAs, HC, HCOC. (G) SOD-like, (H) CAT-like and (I) GPx-like activities of HCOC. (J) Fluorescence images showing intracellular ROS detection by DCFH-DA staining, live/dead staining images and (K) cell viability of L929 cells with different treatments (All groups received 500 μM H 2 O 2 and different HCOC concentrations (I: PBS; II: 0; III: 0.25; IV: 0.50; V: 1.0 mg/mL HCOC). (L) Quantitative analysis of the cells under different treatments. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001) (M) Schematic illustration of pH-responsive activity and ROS scavenging and alleviating cellular oxidative stress of HCOC.

    Techniques Used: Activity Assay, Fluorescence, Staining

    Related Articles

    Staining:

    Article Title: Self-powered mechanoluminescent elastomer for solar-blind ultraviolet emission.
    Article Snippet: .. After stretching, the bacterial solutions were stained using a Live/Dead Bacterial Staining Kit with DMAO and PI (C2030S, Beyotime), which could label live bacteria in green and dead bacteria in red and green. ..

    Article Title: The insect Toll pathway activates antibacterial immunity against the citrus Huanglongbing pathogen
    Article Snippet: .. Following the instructions of the LIVE/DEAD Bacterial Staining Kit with DMAO and PI (Beyotime, C2030S), the co-incubation products were stained. ..

    Article Title: The insect Toll pathway activates antibacterial immunity against the citrus Huanglongbing pathogen.
    Article Snippet: .. Following the instructions of the LIVE/DEAD Bacterial Staining Kit with DMAO and PI (Beyotime, C2030S), the coincubation products were stained. ..

    Article Title: TBP-TA exhibits potent antibacterial activity against vancomycin-resistant Enterococcus.
    Article Snippet: Antimicrobial resistance (AMR) exacerbates bacterial infections in different livestock species, thereby posing major challenges to animal welfare and livestock productivity.. Small-molecule compounds have emerged as a promising strategy for antibacterial therapy, owing to their chemical diversity and unique mechanisms of action.. In this study, we evaluated the antibacterial activity of the small-molecule compound 4-(7-(4-(diphenylamino) phenyl) benzo [c] [1,2,5] thiadiazol-4-yl)-N, N, N-trimethylbenzenaminium (TBP-TA) against vancomycinresistant Enterococcus (VRE) through in vitro antibacterial experiments and in vivo animal models.

    Article Title: Self-powered mechanoluminescent elastomer for solar-blind ultraviolet emission
    Article Snippet: .. After stretching, the bacterial solutions were stained using a Live/Dead Bacterial Staining Kit with DMAO and PI (C2030S, Beyotime), which could label live bacteria in green and dead bacteria in red and green. ..

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. Dulbecco's modified Eagle's medium (DMEM) medium and Roswell Park Memorial Institute (RPMI) 1640 medium containing 1 % penicillin/streptomycin were obtained from Jiangsu KeyGEN Biotech Co., Ltd. DNA Damage Assay Kit by γ-H2AX Immunofluorescence, Beyo3D ROS Assay Kit with DCFH-DA (S1105S), Live/Dead Bacterial Staining Kit with DMAO & PI was obtained to Beyotime Biotechnology (Shanghai) Co., Ltd. Double-mixed injector (suit), MixDS was obtained to Yongqinquan (Suzhou) Intelligent Equipment Co., Ltd. FBS was obtained to Inner Mongolia Jinyuankang Biotechnology Co., Ltd. .. Cell culture plates and 24-well Transwell chamber was obtained from NEST Biotechnology Co., Ltd. (China).

    Article Title: Heat-sealable, hydrophobic, antibacterial bilayer nanocellulose/chitosan film for food preservation.
    Article Snippet: Under the dual challenges of plastic-driven environmental pollution and global food insecurity, there exists an urgent demand for sustainable packaging solutions capable of extending produce shelf life.. Cellulose nanofibers (CNF) and chitosan (CS) are promising bio-based materials; however, their susceptibility to performance loss in high-humidity environments and their lack of intrinsic heat-sealing capability limit practical applications.. Herein, an asymmetric bilayer CNF/CS film crosslinked with genipin is introduced to effectively address the performance degradation under humid conditions.

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.

    Bacteria:

    Article Title: Self-powered mechanoluminescent elastomer for solar-blind ultraviolet emission.
    Article Snippet: .. After stretching, the bacterial solutions were stained using a Live/Dead Bacterial Staining Kit with DMAO and PI (C2030S, Beyotime), which could label live bacteria in green and dead bacteria in red and green. ..

    Article Title: Self-powered mechanoluminescent elastomer for solar-blind ultraviolet emission
    Article Snippet: .. After stretching, the bacterial solutions were stained using a Live/Dead Bacterial Staining Kit with DMAO and PI (C2030S, Beyotime), which could label live bacteria in green and dead bacteria in red and green. ..

    Modification:

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. Dulbecco's modified Eagle's medium (DMEM) medium and Roswell Park Memorial Institute (RPMI) 1640 medium containing 1 % penicillin/streptomycin were obtained from Jiangsu KeyGEN Biotech Co., Ltd. DNA Damage Assay Kit by γ-H2AX Immunofluorescence, Beyo3D ROS Assay Kit with DCFH-DA (S1105S), Live/Dead Bacterial Staining Kit with DMAO & PI was obtained to Beyotime Biotechnology (Shanghai) Co., Ltd. Double-mixed injector (suit), MixDS was obtained to Yongqinquan (Suzhou) Intelligent Equipment Co., Ltd. FBS was obtained to Inner Mongolia Jinyuankang Biotechnology Co., Ltd. .. Cell culture plates and 24-well Transwell chamber was obtained from NEST Biotechnology Co., Ltd. (China).

    Immunofluorescence:

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. Dulbecco's modified Eagle's medium (DMEM) medium and Roswell Park Memorial Institute (RPMI) 1640 medium containing 1 % penicillin/streptomycin were obtained from Jiangsu KeyGEN Biotech Co., Ltd. DNA Damage Assay Kit by γ-H2AX Immunofluorescence, Beyo3D ROS Assay Kit with DCFH-DA (S1105S), Live/Dead Bacterial Staining Kit with DMAO & PI was obtained to Beyotime Biotechnology (Shanghai) Co., Ltd. Double-mixed injector (suit), MixDS was obtained to Yongqinquan (Suzhou) Intelligent Equipment Co., Ltd. FBS was obtained to Inner Mongolia Jinyuankang Biotechnology Co., Ltd. .. Cell culture plates and 24-well Transwell chamber was obtained from NEST Biotechnology Co., Ltd. (China).

    ROS Assay:

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. Dulbecco's modified Eagle's medium (DMEM) medium and Roswell Park Memorial Institute (RPMI) 1640 medium containing 1 % penicillin/streptomycin were obtained from Jiangsu KeyGEN Biotech Co., Ltd. DNA Damage Assay Kit by γ-H2AX Immunofluorescence, Beyo3D ROS Assay Kit with DCFH-DA (S1105S), Live/Dead Bacterial Staining Kit with DMAO & PI was obtained to Beyotime Biotechnology (Shanghai) Co., Ltd. Double-mixed injector (suit), MixDS was obtained to Yongqinquan (Suzhou) Intelligent Equipment Co., Ltd. FBS was obtained to Inner Mongolia Jinyuankang Biotechnology Co., Ltd. .. Cell culture plates and 24-well Transwell chamber was obtained from NEST Biotechnology Co., Ltd. (China).

    Viscosity:

    Article Title: Heat-sealable, hydrophobic, antibacterial bilayer nanocellulose/chitosan film for food preservation.
    Article Snippet: Under the dual challenges of plastic-driven environmental pollution and global food insecurity, there exists an urgent demand for sustainable packaging solutions capable of extending produce shelf life.. Cellulose nanofibers (CNF) and chitosan (CS) are promising bio-based materials; however, their susceptibility to performance loss in high-humidity environments and their lack of intrinsic heat-sealing capability limit practical applications.. Herein, an asymmetric bilayer CNF/CS film crosslinked with genipin is introduced to effectively address the performance degradation under humid conditions.

    Molecular Weight:

    Article Title: Heat-sealable, hydrophobic, antibacterial bilayer nanocellulose/chitosan film for food preservation.
    Article Snippet: Under the dual challenges of plastic-driven environmental pollution and global food insecurity, there exists an urgent demand for sustainable packaging solutions capable of extending produce shelf life.. Cellulose nanofibers (CNF) and chitosan (CS) are promising bio-based materials; however, their susceptibility to performance loss in high-humidity environments and their lack of intrinsic heat-sealing capability limit practical applications.. Herein, an asymmetric bilayer CNF/CS film crosslinked with genipin is introduced to effectively address the performance degradation under humid conditions.

    Starch:

    Article Title: Heat-sealable, hydrophobic, antibacterial bilayer nanocellulose/chitosan film for food preservation.
    Article Snippet: Under the dual challenges of plastic-driven environmental pollution and global food insecurity, there exists an urgent demand for sustainable packaging solutions capable of extending produce shelf life.. Cellulose nanofibers (CNF) and chitosan (CS) are promising bio-based materials; however, their susceptibility to performance loss in high-humidity environments and their lack of intrinsic heat-sealing capability limit practical applications.. Herein, an asymmetric bilayer CNF/CS film crosslinked with genipin is introduced to effectively address the performance degradation under humid conditions.

    Marker:

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.

    Clinical Proteomics:

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.

    Membrane:

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.



    Similar Products

    99
    Beyotime dmao pi bacterial live
    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. <t>(J)</t> <t>Live/dead</t> staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
    Dmao Pi Bacterial Live, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc13011198-324-5-10
    Average 99 stars, based on 1 article reviews
    dmao pi bacterial live - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Beyotime live dead bacterial staining kit
    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. <t>(J)</t> <t>Live/dead</t> staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
    Live Dead Bacterial Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc13059000-90-11-18
    Average 99 stars, based on 1 article reviews
    live dead bacterial staining kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology live dead bacterial staining kit
    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. <t>(J)</t> <t>Live/dead</t> staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
    Live Dead Bacterial Staining Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/cell+dead+kit+live+staining/pmc12906132-97-11-15
    Average 86 stars, based on 1 article reviews
    live dead bacterial staining kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    Beyotime dmao pi
    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. <t>(J)</t> <t>Live/dead</t> staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
    Dmao Pi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc12805112-73-45-51
    Average 99 stars, based on 1 article reviews
    dmao pi - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Beyotime dead bacterial staining kit
    Cytotoxicity and cytology evaluations of the Co-BOS@C/F Gel. Cell viability with different hydrogel treatment in (a) L929 cells and (b) HUVECs at different concentrations for 24 h and 48 h; Fluorescence images <t>of</t> <t>live/dead</t> cells of HUVECs using a Calcein-AM/PI assay. The cells were co-cultured with the media extracts of different hydrogels, incubated for (c) 1 D and (d) 3 D. (e) Scratch assay for HUVECs treated with C/F Gel, BOS@C/F Gel, and Co-BOS@C/F Gel. Data are presented as mean value ± SD. ( ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; NS, not significant).
    Dead Bacterial Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc12805112-73-40-51
    Average 99 stars, based on 1 article reviews
    dead bacterial staining kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Beyotime live dead bacterial viability kit
    Cytotoxicity and cytology evaluations of the Co-BOS@C/F Gel. Cell viability with different hydrogel treatment in (a) L929 cells and (b) HUVECs at different concentrations for 24 h and 48 h; Fluorescence images <t>of</t> <t>live/dead</t> cells of HUVECs using a Calcein-AM/PI assay. The cells were co-cultured with the media extracts of different hydrogels, incubated for (c) 1 D and (d) 3 D. (e) Scratch assay for HUVECs treated with C/F Gel, BOS@C/F Gel, and Co-BOS@C/F Gel. Data are presented as mean value ± SD. ( ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; NS, not significant).
    Live Dead Bacterial Viability Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pm41943295-122-14-19
    Average 99 stars, based on 1 article reviews
    live dead bacterial viability kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Beyotime live dead staining kit
    In vitro antibacterial activity of materials. ( A ) Macroscopic photographs of spread plates fo r S. aureus grown on various samples; ( B ) Inhibition rates of various groups against S. aureus determined by spread plate assays, with the SP group serving as the control; ( C ) Macroscopic photographs of spread plates fo r E. coli grown on various samples; ( D ) Inhibition rates of various groups against E. coli determined by spread plate assays, with the SP group serving as the control; ( E ) Crystal violet staining images of S. aureus biofilms cultured in sample eluates; ( F ) Biofilm quantification via crystal violet staining, with data expressed as absorbance at 570 nm after ethanol solubilization. ( G ) SEM images of bacteria and biofilms on the surfaces of each sample group. ( H <t>)</t> <t>Live/dead</t> staining images of biofilms from each group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
    Live Dead Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+bacterial+staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc13033999-104-12-16
    Average 99 stars, based on 1 article reviews
    live dead staining kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. (J) Live/dead staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

    Journal: Bioactive Materials

    Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

    doi: 10.1016/j.bioactmat.2026.02.043

    Figure Lengend Snippet: Characterization, and Cytocompatibility Validation of HCOC. (A) Schematic illustration of the development of HCOC. (B) FTIR spectrum of OSA, CMCS and OC hydrogel. (C) Time-dependent evolution of gelation of OC and HCOC. (D) SEM images of HCOC and EDS mapping images of C, N, O and Cu for HCOC. (E) FTIR spectra of HC, OC and HCOC. (F) Dynamic frequency sweep measurements of OC and HCOC. (G) Frequency-dependent viscoelastic behavior of OC and HCOC. (H) Alternating strain sweep with alternating strains of 1% and 1000% at 100s intervals and (I) Self-healing behavior of HCOC. (J) Live/dead staining showing the metabolic activity of L929 and RAW 264.7 cells after treatment with HCOC for 48 h. Rates of proliferation of (K) L929 cells and (L) RAW 264.7 cells after treatment with PBS or HCOC. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

    Article Snippet: Following the protocol of the DMAO/PI Bacterial Live/Dead Staining Kit (Beyotime Biotechnology), the bacteria were incubated with a working solution containing both DMAO and PI dyes in the dark at room temperature for 15-20 min. Fluorescence microscopy imaging was subsequently carried out.

    Techniques: Biomarker Discovery, Staining, Activity Assay

    pH Self-Adaptive Antioxidant Capacity of HCOC (Stage II: anti-inflammation). Cu ion release behavior of (A) HC (1 mg/mL) and (B) HCOC (1 mg/mL) at different pH levels. (C) ABTS + and (D) H 2 O 2 scavenging activity at different pH of Cu 5.4 O, HC and HCOC. (E) O 2 ∙ - , (F)∙OH scavenging activity of Cu 5.4 O, HAs, HC, HCOC. (G) SOD-like, (H) CAT-like and (I) GPx-like activities of HCOC. (J) Fluorescence images showing intracellular ROS detection by DCFH-DA staining, live/dead staining images and (K) cell viability of L929 cells with different treatments (All groups received 500 μM H 2 O 2 and different HCOC concentrations (I: PBS; II: 0; III: 0.25; IV: 0.50; V: 1.0 mg/mL HCOC). (L) Quantitative analysis of the cells under different treatments. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001) (M) Schematic illustration of pH-responsive activity and ROS scavenging and alleviating cellular oxidative stress of HCOC.

    Journal: Bioactive Materials

    Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

    doi: 10.1016/j.bioactmat.2026.02.043

    Figure Lengend Snippet: pH Self-Adaptive Antioxidant Capacity of HCOC (Stage II: anti-inflammation). Cu ion release behavior of (A) HC (1 mg/mL) and (B) HCOC (1 mg/mL) at different pH levels. (C) ABTS + and (D) H 2 O 2 scavenging activity at different pH of Cu 5.4 O, HC and HCOC. (E) O 2 ∙ - , (F)∙OH scavenging activity of Cu 5.4 O, HAs, HC, HCOC. (G) SOD-like, (H) CAT-like and (I) GPx-like activities of HCOC. (J) Fluorescence images showing intracellular ROS detection by DCFH-DA staining, live/dead staining images and (K) cell viability of L929 cells with different treatments (All groups received 500 μM H 2 O 2 and different HCOC concentrations (I: PBS; II: 0; III: 0.25; IV: 0.50; V: 1.0 mg/mL HCOC). (L) Quantitative analysis of the cells under different treatments. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001) (M) Schematic illustration of pH-responsive activity and ROS scavenging and alleviating cellular oxidative stress of HCOC.

    Article Snippet: Following the protocol of the DMAO/PI Bacterial Live/Dead Staining Kit (Beyotime Biotechnology), the bacteria were incubated with a working solution containing both DMAO and PI dyes in the dark at room temperature for 15-20 min. Fluorescence microscopy imaging was subsequently carried out.

    Techniques: Activity Assay, Fluorescence, Staining

    Cytotoxicity and cytology evaluations of the Co-BOS@C/F Gel. Cell viability with different hydrogel treatment in (a) L929 cells and (b) HUVECs at different concentrations for 24 h and 48 h; Fluorescence images of live/dead cells of HUVECs using a Calcein-AM/PI assay. The cells were co-cultured with the media extracts of different hydrogels, incubated for (c) 1 D and (d) 3 D. (e) Scratch assay for HUVECs treated with C/F Gel, BOS@C/F Gel, and Co-BOS@C/F Gel. Data are presented as mean value ± SD. ( ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; NS, not significant).

    Journal: Bioactive Materials

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing

    doi: 10.1016/j.bioactmat.2025.12.049

    Figure Lengend Snippet: Cytotoxicity and cytology evaluations of the Co-BOS@C/F Gel. Cell viability with different hydrogel treatment in (a) L929 cells and (b) HUVECs at different concentrations for 24 h and 48 h; Fluorescence images of live/dead cells of HUVECs using a Calcein-AM/PI assay. The cells were co-cultured with the media extracts of different hydrogels, incubated for (c) 1 D and (d) 3 D. (e) Scratch assay for HUVECs treated with C/F Gel, BOS@C/F Gel, and Co-BOS@C/F Gel. Data are presented as mean value ± SD. ( ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; NS, not significant).

    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) medium and Roswell Park Memorial Institute (RPMI) 1640 medium containing 1 % penicillin/streptomycin were obtained from Jiangsu KeyGEN Biotech Co., Ltd. DNA Damage Assay Kit by γ-H2AX Immunofluorescence, Beyo3DTM ROS Assay Kit with DCFH-DA (S1105S), Live/Dead Bacterial Staining Kit with DMAO & PI was obtained to Beyotime Biotechnology (Shanghai) Co., Ltd. Double-mixed injector (suit), MixDS was obtained to Yongqinquan (Suzhou) Intelligent Equipment Co., Ltd. FBS was obtained to Inner Mongolia Jinyuankang Biotechnology Co., Ltd.

    Techniques: Fluorescence, Cell Culture, Incubation, Wound Healing Assay

    In vitro antibacterial activity of materials. ( A ) Macroscopic photographs of spread plates fo r S. aureus grown on various samples; ( B ) Inhibition rates of various groups against S. aureus determined by spread plate assays, with the SP group serving as the control; ( C ) Macroscopic photographs of spread plates fo r E. coli grown on various samples; ( D ) Inhibition rates of various groups against E. coli determined by spread plate assays, with the SP group serving as the control; ( E ) Crystal violet staining images of S. aureus biofilms cultured in sample eluates; ( F ) Biofilm quantification via crystal violet staining, with data expressed as absorbance at 570 nm after ethanol solubilization. ( G ) SEM images of bacteria and biofilms on the surfaces of each sample group. ( H ) Live/dead staining images of biofilms from each group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Simvastatin and Moxifloxacin Co-Delivery via ZIF-8/PDA Coating on PEEK Implants: A Strategy for Combating Implant-Associated Infection and Enhancing Osseointegration

    doi: 10.2147/IJN.S586499

    Figure Lengend Snippet: In vitro antibacterial activity of materials. ( A ) Macroscopic photographs of spread plates fo r S. aureus grown on various samples; ( B ) Inhibition rates of various groups against S. aureus determined by spread plate assays, with the SP group serving as the control; ( C ) Macroscopic photographs of spread plates fo r E. coli grown on various samples; ( D ) Inhibition rates of various groups against E. coli determined by spread plate assays, with the SP group serving as the control; ( E ) Crystal violet staining images of S. aureus biofilms cultured in sample eluates; ( F ) Biofilm quantification via crystal violet staining, with data expressed as absorbance at 570 nm after ethanol solubilization. ( G ) SEM images of bacteria and biofilms on the surfaces of each sample group. ( H ) Live/dead staining images of biofilms from each group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

    Article Snippet: Subsequently, the biofilm on the sample surface was stained using a bacterial live/dead staining kit (DMAO/PI; Beyotime, China) according to the manufacturer’s instructions.

    Techniques: In Vitro, Activity Assay, Inhibition, Control, Staining, Cell Culture, Bacteria

    Biocompatibility of the materials. ( A ) Live/dead staining images of MC3T3-E1 cells co-cultured on the surface of each group of samples; ( B ) Quantitative analysis of live/dead staining of samples from each group; ( C ) Proliferation curves of MC3T3-E1 cells in each group, the data show the OD value at 450 nm obtained using the CCK-8 method; ( D ) SEM images of MC3T3-E1 cells on the surface of each group of samples; ( E ) Cytoskeletal staining CLSM images of MC3T3-E1 cells in each group.

    Journal: International Journal of Nanomedicine

    Article Title: Simvastatin and Moxifloxacin Co-Delivery via ZIF-8/PDA Coating on PEEK Implants: A Strategy for Combating Implant-Associated Infection and Enhancing Osseointegration

    doi: 10.2147/IJN.S586499

    Figure Lengend Snippet: Biocompatibility of the materials. ( A ) Live/dead staining images of MC3T3-E1 cells co-cultured on the surface of each group of samples; ( B ) Quantitative analysis of live/dead staining of samples from each group; ( C ) Proliferation curves of MC3T3-E1 cells in each group, the data show the OD value at 450 nm obtained using the CCK-8 method; ( D ) SEM images of MC3T3-E1 cells on the surface of each group of samples; ( E ) Cytoskeletal staining CLSM images of MC3T3-E1 cells in each group.

    Article Snippet: Subsequently, the biofilm on the sample surface was stained using a bacterial live/dead staining kit (DMAO/PI; Beyotime, China) according to the manufacturer’s instructions.

    Techniques: Staining, Cell Culture, CCK-8 Assay